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The reaction mixtures were prepared in 384 well plate using a Freedom Evo robot (Tecan, M Switzerland) and run in an ABI 7900HT sequence detection system (Applied Biosystems) using the following conditions: 50 for 2 95 for 10 followed by 45 cycles of 95 for 15 and then 60 for 1 after which dissociation curves were established.

Made a lot of money and well, that's pretty much it, folks? Moore's Law is still hanging on, but only by a thread it's gone from a hardware problem to an immensely difficult software problem. If prices open between nine and eleven dollars (given the private equity placement of 97 million shares and Scorpio's filing for a 100 million dollar IPO) this would be equitable.

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